Mechanism of GTPγS-dependent regulation of smooth muscle contraction
نویسندگان
چکیده
منابع مشابه
Potential mechanism of cyclosporine A-induced vascular smooth muscle contraction.
Arterial hypertension is a common side effect of cyclosporine A therapy; however, the cellular mechanism of cyclosporine A-induced hypertension is still unknown. The present study, therefore, examined the effect of cyclosporine A on Ca2+ kinetics and contraction in primary cultures of vascular smooth muscle cells. Cyclosporine A (10 micrograms/ml) did not affect resting intracellular free Ca2+ ...
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Photolysis of caged calcium (Nitr5, Calbiochem®) can be used to studycalcium dependent processes such as excitation-contraction coupling andmuscular mechanics. Expensive high energy light sources are routinely used forUV light exposure; this study describes an alternative low cost Xenon flash unitconstmcted in our laboratOlY. A 300 J short arc Xenon flash lamp (Heimarl1l®)wa...
متن کاملMechanism of the phosphorylation-dependent regulation of smooth muscle heavy meromyosin.
Phosphorylation of smooth muscle heavy meromyosin (HMM) has been shown to result in about a 25-fold increase in the steady-state Vmax of the actin-activated MgATPase activity from 0.07 s-1 for unphosphorylated HMM to 1.9 s-1 for phosphorylated HMM. The steady-state MgATPase activity of unphosphorylated HMM in the absence of actin is 0.004 s-1. The true extent of regulation might be even larger ...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1992
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)60082-x